Scientists find the ability to compute molecular properties vital when utilizing and synthesizing peptoids and peptides for study. These properties include isoelectric points, molecular weights and extinction coefficients.
Two web utilities are presented in a suite- peptide calculator and peptoid calculator. Using both tools, scientists can compute the correct chemical formulae for the peptoid/peptide as well as molecular weight. At the same time, the assignment of mass spectral leaks can be done automatically, just as protecting group adducts and deletion sequences.
The peptide calculator similarly gives a calculated molar extinction coefficient, isoelectric point, strand congruity profile and peptide charge summary in a graph. The calculator indicates probable regions of synthesis difficulty. Peptoid calculator presents an unprecedented resource for scientists in this field on top of the automatic spectral assignments that come with the two utilities.
In fact, many researchers predict that Pep-calc.com will be an invaluable tool for people in the application or/and synthesis of peptoids and peptides. This is especially true because of its ever-increasing database of more than 120 different amino acids, natural building blocks of peptoids and synthetic peptide building blocks.
Scientists use peptides to map antibody epitopes, manufacture epitope-particular antibodies, design novel enzymes and develop enzyme binding sites, vaccines and drugs. In the past, the synthesis of peptide was a labor-intensive operation.
Additionally, it produced small yields. Today, there is an increase in peptide chemistry, which leads to improved production methods. For this reason, researchers have more access to peptide synthesis for applications of general research.
There has also been an increased optimization and mass production of peptide synthesis; the procedure of manufacturing peptides is far from perfect. The truncation and total deletion of sequences and isomers is one drawback. This is because of reaction with free protecting groups and incomplete deprotection.
These undesirable events can rear their ugly head at any point of peptide synthesis. For this reason, the probability of something negatively affecting the procedure increases with the increase in peptide sequence. It is correct to say that peptide yield is inversely proportional to the length of peptide.